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Donnish Journal of Agricultural Research

October 2014 Vol. 1(1), pp. 001-004

Copyright 2014 Donnish Journals




Original Research Article


Measuring the Biodiversity in Iraqi Sheep Breeds Using Microsatellites: Detection of Silent and Private Alleles


*Talib Ahmed Jaayid and Jaafer Mohammed Owaid

Genetic Engineering Laboratory, College of Agriculture, University of Basrah (UOB), Basrah 320, Iraq.

Corresponding Author E-mail: taleb1968@yahoo.com

Accepted 4th October, 2014.



Abstract


This study was conducted in the genetic engineering lab., college of agriculture, university of Basrah (UoB). Four Iraqi breeds were included in this study, Arabi, Awasi, Hamdani, and Karadi, twenty Blood samples were collected from each breed. DNA was extracted using a kit from Invitrogen company, U.S.A, using Four microsatellite markers (ILSTS005, OarAE129, OARCP34 and CSSM31) to detect the silent and private alleles. A total of 166 and 25 silent and private alleles was obtained for the four molecular markers, respectively. The larger number of silent alleles (51) was observed with CSSM31 molecular marker, while the lower number of silent alleles (28) was recorded with the marker OARCP34 . The number of private alleles with different sizes were 23 alleles for all molecular markers. The larger number of private alleles (8) was with ILSTS005 molecular marker, while the lower number of private alleles (5) was detected with OARCP34 molecular marker, so it's possible to say that we can use OARCP34 molecular marker in biodiversity studies of Iraqi sheep breeds.

Keywords: Microsatellites technique, Iraqi sheep breeds, silent allele, private allele, genetic polymorphism.

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Cite This Article:

Talib Ahmed Jaayid and Jaafer Mohammed Owaid. Measuring the Biodiversity in Iraqi Sheep Breeds Using Microsatellites: Detection of Silent and Private Alleles. Donnish Journal of Agricultural Research 1(1) 2014 pp. 001-004.


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