Donnish Journal of Agricultural Research

March 2015 Vol. 2(3), pp. 020-026

Copyright © 2015 Donnish Journals

Original Research Article

Comparison of Rhizoctonia solani Isolated from Soil in Baghdad – Iraq Genetically with World Isolates

T. A. Kareem and M. S. Hassan

Department of Plant protection, College of Agriculture, University of Baghdad, Iraq

Corresponding Author E-mail: tariqask@yahoo.com

Accepted 24th February, 2015.

Abstract

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R. solani is a soil-borne Basidiomycete occurring worldwide. The objective of this study was to compare isolates of Rhizoctonia solani from some soil samples collected from different regions (Abu Ghraib, Yusifiyah, Al Rashidiya, Tarmiya, Doura and Quraiat) in Baghdad – Iraq. The degree of kinship for R. solani isolates was estimated by the PCR technology. A total of 24 isolates of R. solani were isolated from 114 samples of soil. Pathogenicity test using radish seedlings showed that 11 isolates were strong virulent, 5 isolates were moderately virulent, and 8 isolates showed low virulence. The colony morphology on PSA of isolates (IQ4, IQ6, IQ9, IQ14, IQ15, IQ16, IQ24, IQ25, IQ26, IQ28 and IQ50) showed typical features of R. solani. The sequences of 9 R. solani isolates from tomato plants deposited at NCBI – GenBank nucleotide database. The GenBank accession number for each isolate sequence was available since November 2013 as KF372663 – KF372673. The results of phylogenetic analysis showed that, identifying soil isolates were within AG4: two isolates, IQ4 and IQ6 were closely related to AG4-HGI and 9 isolates, IQ14, IQ15, IQ50, IQ9, IQ16, IQ24, IQ26, IQ25 and IQ28 were belonged to AG4–HG III (BS = 99%) and showed sequence similarity of 94, 97, 85, 96, 67, 69, 60, 74, 66, 66 and 65% respectively.

Keywords: Rhizoctonia solani, soil, ITS, PCR.

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Cite This Article:

T. A. Kareem and M. S. Hassan. Comparison of Rhizoctonia solani Isolated from Soil in Baghdad – Iraq Genetically with World Isolates. Donnish Journal of Agricultural Research 2(3) 2015 pp. 020-026.