Donnish Journal of Biomedical Research (DJBR)
August 2015 Vol. 2(1), pp. 001-006
Copyright © 2015 Donnish Journals
Original Research Paper
RECK Gene Polymorphisms in Hepatocellular Carcinoma and Cirrhotic Patients Related to Hepatitis C Virus
Ingy Badawy1*, Marwa K. Darwish2, Omar Samir1, Yassin Baraqouni1, Mohamed Mahmoud Nassef1, Hossam E. Elshafey3, Seham M. Mahmoud4 and Hala H. El Deeb5
1Faculty of Biotechnology, Misr University for Science and Technology, Cairo, Egypt.
2Faculty of Science, Suez University, Suez, Egypt.
3Surgery Department, National Hepatology and Tropical Medicine Institute, Cairo, Egypt.
4Tropical Medicine Department, El Sahel Teaching Hospital, Cairo, Egypt.
5Clinical Pathology Department, El Sahel Teaching Hospital, Cairo, Egypt.
Corresponding Author: Dr. Ingy Badawy
Accepted 27th July, 2015.
Abstract
Background: Hepatocellular carcinoma (HCC) is the sixth most common cancer globally. HCC is the third most common cause of cancer mortality. The development of HCC is a multistep and complex process. Multiple environmental risk factors, including chronic hepatitis B virus (HBV) or hepatitis C virus (HCV) infection, cirrhosis, carcinogen exposure, and a variety of genetic factors contribute to hepatocarcinogenesis. The reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) down-regulation has been confirmed in numerous human cancers and is clinically associated with metastasis. This study investigates the potential associations of RECK single-nucleotide polymorphisms (SNPs) with HCC susceptibility and clinicopathologic characteristics in HCV Egyptian patients. Methodology: This study was conducted on a total number of 110 participants admitted to National Hepatology & Tropical Medicine Research Institute. The participants of this study were divided into three groups (30 HCV patients, 30 HCC patients and 30 liver cirrhosis (LC) patients) in addition to 20 healthy individuals as control group were analyzed for RECK SNP (rs16932912) genotyping using real-time. Results: RECK rs16932912 mutant genotypes GG/AG showed no significant value in HCC compared to wild type (P= 0.373). Mutant genotype was higher in liver cirrhosis group than other groups (P=0.001). High significant levels of ALT, AST, AFP, ALB (P=<0.01) among the rs16932912 mutant AG/AA genotypes versus wild GG genotype. Conclusion: Our study showed that the presence of A/G genotype was associated with prognosis in HCV-HCC and HCV-LC patients.
Keywords: RECK gene, HCV, HCC, LC.
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Cite This Article:
Ingy Badawy, Marwa K. Darwish, Omar Samir, Yassin Baraqouni, Mohamed Mahmoud Nassef, Hossam E. Elshafey, Seham M. Mahmoud and Hala H. El Deeb. RECK Gene Polymorphisms in Hepatocellular Carcinoma and Cirrhotic Patients Related to Hepatitis C Virus. Donnish Journal of Biomedical Research 2(1) 2015 pp. 001-006.
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