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Donnish Journal of Cancer Research and Experimental Oncology

December 2016 Vol. 2(2), pp. 004-011

ISSN: 2984-8970

Copyright 2016 Donnish Journals




Original Research Paper


MicroRNA-143 and -155 Levels as Biomarkers for Hepatocellular Carcinoma in Patients with Chronic Hepatitis C Virus Infection


Mohamed A. Saleh1, Azza M. Faried2, Mai Ismail Mehrez1, Sahar Mohamed Mustafa2, Hala H. El Deeb3, Naglaa Adly Abdelazeem4, Anwar El Reweny5 and Mohamed Fawzi6

1Internal Medicine Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
2Hepatology Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
3Clinical Pathology Department, El Sahel Teaching Hospital, Egypt.
4MedicalBiochemistry Department, Beni-Suef Faculty of Medicine, Egypt.
5Clinical pathology Department, Damanhur National Medical Institute, Egypt.
6Radiology Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.

Accepted 10th November, 2016.



Abstract


Background: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. It commonly develops on cirrhotic livers, and surveillance programs have therefore been suggested to identify early HCC, at a stage when it remains suitable for surgical therapy and has a better clinical outcome. Aim: This study aimed to investigate the association between MicroRNAs 143, 155 and prediction of HCC in Egyptian patients. Methods: The present study was done on a total number 141 included 47 healthy individuals as control group. Serum samples were obtained from 94 patients with chronic liver disease, divided into two groups: Group (I) included forty-seven patients with HCC, patients with cancers other than HCC or metastatic liver cancer were excluded. Group (II) included forty-seven patients with liver cirrhosis and without any evidence of HCC. Questionnaire was used to collect clinical data while Alpha-fetoprotein (AFP), serum HBsAg and anti-HCV were determined using ELISA method. The expression of miRNA was evaluated using real-time quantitative RT-PCR. Abdominal ultrasound scan was also done. Results: The ROC curve analysis revealed that both serum miR-143 and miR-155 could serve as valuable biomarkers for detection of HCC from healthy controls. The highest AUC to differentiate HCC patients from non-HCC was 0.804 for miR-143 and 0.885 for miR-155. Conclusion: This study demonstrated the beneficial role of miR-155 expression might be further evaluated as novel noninvasive diagnostic biomarkers for HCC more thanmiR-143.

Keywords: Hepatocellular carcinoma (HCC), Liver Cirrhosis (LC), Alpha-Fetoprotein (AFP), MicroRNAs (miRNA).

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Cite This Article:

Mohamed A. Saleh, Azza M. Faried, Mai Ismail Mehrez, Sahar Mohamed Mustafa, Hala H. El Deeb, Naglaa Adly Abdelazeem, Anwar El Reweny and Mohamed Fawzi. MicroRNA-143 and -155 Levels as Biomarkers for Hepatocellular Carcinoma in Patients with Chronic Hepatitis C Virus Infection. Donnish Journal of Cancer Research and Experimental Oncology 2(2) 2016 pp. 004-011.


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