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Donnish Journal of Dentistry and Oral Hygiene

November 2015 Vol. 1(4), pp. 016-020

Copyright 2015 Donnish Journals




Original Research Paper


Effectiveness of Lime (Citrus aurantifolia) Extract Solution in Inhibiting Bacteria Streptococcus Mutans Case of Early Childhood Caries


Fajriani1* and Mahrum2

1Department of Pediatric, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.
2Student, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia.

Corresponding Author's E-mail: fajriani_fkg@yahoo.com

Accepted 11th October, 2015.



Abstract


Background: Early childhood caries is a dental health problem found in children. Mutans streptococci is a bacteria that causes dental caries. Lime fruit has essential oils which have an active antibacterial compound. Aim: This study aimed to see the effect of lime extract 40% for the growth of mutans streptococci in children with early childhood caries. Materials and Methods: This study used a cross-sectional study design and used experiments with pretest-posttest control group design. The study sample comprises 60 children who fit with the criteria. 30 subjects as a control group (rinsed with distilled water) and 30 subjects as a treatment group (rinsed with lime extract 40%). Each sample was given the same intervention, the first step was the collection of saliva before intervention (pretest), in the second step, subjects were given 10 ml of lime extract 40% to rinse their mouth for about 30 seconds. After that, saliva was collected twice in 15 minutes (post 1) and 30 minutes (post 2) after rinse. Furthermore, saliva was taken to the laboratory to count the number of colonies of mutans streptococci bacteria using the Colony Counter method and measured in CFU (Colony Forming Unit). Data processing and analysis was done using the SPSS 22.0 software for windows version. Results: The results of repeated ANOVA and paired t-test showed a significant reduction of the number of mutans streptococci colonies from before rinse to 30 minutes after rinse with lime extract 40%. The number of bacterial colonies on the pre was 72.23 CFU, on post 1 as 103.36 CFU, and the post 2 was 14.03 CFU. Statistical value showed p= 0.000, that means the reduction of mutans streptococci colonies was significant. Conclusion: Lime extract 40% is effective to reduce the growth of mutans streptococci colonies in the mouth.

Keywords: Lime extract, Essential oils, Streptococcus mutans, Caries.

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Cite This Article:

Fajriani and Mahrum. Effectiveness of Lime (Citrus aurantifolia) Extract Solution in Inhibiting Bacteria Streptococcus Mutans Case of Early Childhood Caries. Donnish Journal of Dentistry and Oral Hygiene 1(4) 2015 pp. 016-020.


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