SUBMIT MANUSCRIPT

Donnish Journal of Microbiology and Biotechnology Research

June 2015 Vol. 2(1), pp. 001-007

Copyright 2015 Donnish Journals




Original Research Paper


Epidemiological and Molecular Study for Distribution of Cryptosporidium parvum in Diarrheic Children in Al-Muthanna Province, Iraq


Muhammed Oda Mallah* and Nouran Raad Jomah

College of Science, Al-Muthanna University, Iraq.

Corresponding Author E-mail: muhammed_mallah@yahoomail.com

Accepted 12th May, 2015.



Abstract


This study was carried out in AL-Muthanna province during the period from October 2013 to May 2014 in the Educational AL-Hussein hospital in AL-Samawa, General- AL-Rumaiytha hospital, Feminine and Children's hospital in AL-Samawa and AL-Rumaiytha health centers. A total of 100 stool samples were taken from children aged 1 month to less than 10 years who were suffering from acute or persistent diarrhea examined by Ziehl-Neelsen stain and RT- PCR assay. In this study, a total of 100 fecal samples of children were examined by Ziehl-Neelsen stain, out of which 21 fecal samples had Cryptosporidium parvum with 21%. The results showed oocysts stained red bodies against a dark blue background, with a clear halo around the oocyst. Also, the present study recorded non significant differences at level (P<0.05) in all the age groups between male and female., the highest rate of the infection in male was 42.86% compared with female (23.80%) in the age group (1-3) years old. The results showed significant differences at level (P<0.05) between rural and urban regions in age groups (1-3) years old compared with other age groups, the highest rate of infection in rural regions (47.62%) compared with urban (19.05%). Also this study recorded a total of 100 fecal samples of children examined by real time PCR out of which 18 fecal samples had found Cryptosporidium parvum with 18%. The present study showed non significant differences at level (P<0.05) between male and female in all age groups, the highest rate of the infection in male was 44.44% compared with female (22.22%) in the age group (1-3) years old. Also the results showed significant differences at level (P<0.05) between rural and urban regions in all age groups compared with other age groups except (7-9) years old. The highest rate of infection in rural regions was 50.5% compared with urban (16.67%).

Keywords: C. parvum, Children, Ziehl-Neelsen, RT-PCR, Iraq.

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Cite This Article:

Muhammed Oda Mallah and Nouran Raad Jomah. Epidemiological and Molecular Study for Distribution of Cryptosporidium parvum in Diarrheic Children in Al-Muthanna Province, Iraq. Donnish Journal of Microbiology and Biotechnology Research 2(1) 2015 pp. 001-007.


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