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Donnish Journal of Microbiology and Biotechnology Research

August 2016 Vol. 3(2), pp. 008-012

ISSN: 2984-8679

Copyright 2016 Donnish Journals




Original Research Paper


Phytochemical Constituents and Comparative in vitro Antibacterial Activity of Leaf and Stem Extracts of Pneumatopteris afra (Christ) Holttum


Adebiyi, Adedeji Olayinka1* and David, Oluwole Moses2

1Department of Plant Science and Biotechnology, Ekiti State University, Ado-Ekiti, Nigeria.
2Department of Microbiology, Ekiti State University, Ado-Ekiti, Nigeria.

Corresponding Author's Email: djadebiyi@yahoo.com

Accepted 7th July, 2016.



Abstract


Ferns (pteridophytes) have been reported to have different phytochemicals. They have been used in the treatment of many infections. The phytochemical analyses and antibacterial activities of Pneumatopteris afra were investigated in this study. Standard physical-chemical methods were used to determine the phytochemical constituents of the leaf and the stem of the P. afra. Disc diffusion dilution method was used to determine the antibacterial activities of the ethanolic, ethyl acetate and n-hexane extracts of the leaf and stem of the plant. Escherichia coli ATCC 8739 did not produce the zone of inhibition when exposed to the n-hexane extracts of the plant. Unlike ethanolic extract, the n-hexane extract of the leaf of P. afra performed better than the stem extract. Ethyl acetate extract of the stem performed better than the leaf extract. Except for organisms, Serratia marcescens ATCC 9986 and Shigella sonnei ATCC 29930 all the test organisms showed more susceptibility to ethyl acetate extract of the stem. In vivo evaluation of the extract and possible mechanism(s) of action of the extracts of the plant is still open to investigation.

Keywords: Pneumatopteris afra, Ferns, Antibacterial, Phytochemical.

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Cite This Article:

Adebiyi, Adedeji Olayinka1* and David, Oluwole Moses. Phytochemical Constituents and Comparative in vitro Antibacterial Activity of Leaf and Stem Extracts of Pneumatopteris afra (Christ) Holttum. Donnish Journal of Microbiology and Biotechnology Research 3(2) 2016 pp. 008-012.


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