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Donnish Journal of Microbiology and Biotechnology Research

April 2017 Vol. 4(3), pp. 010-023

Copyright 2017 Donnish Journals




Original Research Paper


Genetic Variation of Enterobacter cloacae Using Random Amplified Polymorphic DNA (RAPD-PCR) Technique


Yahya A. Abbas1* and Ghosoon Fadhel Radhi2

1Nassiriya Technical Institute, Southern Technical University, Iraq.
2Department of Biology, College of Science, University of Basrah, Iraq.

Accepted 20th February, 2017.



Abstract


The genetic variation of 40 isolates of Enterobacter cloacae was studied using four primers (640, 650, OPAR3, OPAR8) by RAPD-PCR technique. Most isolates gave DNA bands different in numbers and sizes. Among the primers (640) exhibited the highest efficacy reaching (322) total bands. The genetic diversity relationships between Enterobacter cloacae were generated by UPGMA analysis. Genetic similarity ranged from 0.58 (low similarity) to 0.93 (high similarity). The dendrogram differentiated the species into 31 distinct clusters at similarity level which ranged from (0.84-0.93).

Keywords: RAPD, Enterobacter cloacae, Genetic variation

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Cite This Article:

Yahya A. Abbas and Ghosoon Fadhel Radhi. Genetic Variation of Enterobacter cloacae Using Random Amplified Polymorphic DNA (RAPD-PCR) Technique. Donnish Journal of Microbiology and Biotechnology Research 4(3) 2017 pp. 010-023.


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