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Donnish Journal of Microbiology and Biotechnology Research

February 2017 Vol. 4(2), pp. 004-009

Copyright 2017 Donnish Journals




Original Research Paper


Isolation and Identification of Bacteria from Diabetic and Non-diabetic Patients with Periodontitis


Amani A. Al-Abdul1* and Israa K. Hussein2

1Department of Biology, College of Sciences, University of Basrah, Iraq.
2Bacteriology Laboratory of Al-shifa Hospital Basrah, Iraq.

Corresponding Author's Email: amani_rd1975@yahoo.com

Accepted 20th January, 2017.



Abstract


This study included isolation and identification of periodontopathogenic bacteria from periodontal pocket with depth > 4mm from diabetic patients (both insulin dependent and non-insulin dependent) and non-diabetic patients coming under the age group of 15-73 years and all having periodontitis from AL-Shaheed Qais Specialized dental teaching center, AL-Juniana Specialized dental teaching center, Dental teaching clinics in the college of dentistry, AL-Juniana center for primary health care, 60 street center for primary health care and AL-Nahda center for primary health care in the center of Basrah governorate of Iraq. The study was conducted during the period from September 2015 to May 2016. The sample chosen for this study, gingival crevicular fluid (GCF) was collected by absorbent paper points from a total of 46 patients which comprised 21(45.65%) diabetic patients and 25(54.35%) non-diabetic patients, including 29 males and yielded the isolation of 97 bacterial isolates divided into to 44 bacterial isolates that represented (47.72%) from diabetic patients and 53 bacterial isolates that represented (47.16%) from non-diabetic patients. Bacteriological investigation of 92 bacterial isolates belonging to 26 species of 14 genera led to the identification of anaerobic bacteria 6(13.6%) in the diabetic patients and 4(7.5%) in the non-diabetic patients and 85 aerobic bacteria confirmed by automated microbiological system Vitek2. The results of this study showed that Staphylococcus spp was more common among isolates from two groups and represented 13(29.5%) in the diabetic group and 27(50.9%) in non-diabetic group, followed by Enterobacteriaceae that represented (27.3%), (13.2%) in diabetic and non-diabetic patients respectively. While Streptococcus spp represented 8(18.2%) in diabetics and 4(7.5%) in non-diabetic, Leuconostoc spp represented 4(9.1%) in diabetics and 6(11.3%) in non-diabetic.

Keywords: Periodontitis, Oral flora, Diabetes mellitus.

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Cite This Article:

Amani A. Al-Abdul and Israa K. Hussein. Isolation and Identification of Bacteria from Diabetic and Non-diabetic Patients with Periodontitis. Donnish Journal of Microbiology and Biotechnology Research 4(2) 2017 pp. 004-009.


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