SUBMIT MANUSCRIPT

Donnish Journal of Medical Laboratory and Diagnosis

October 2015 Vol. 1(2), pp. 010-014

Copyright 2015 Donnish Journals




Original Research Paper


Amniocentesis for Fetal Karyotyping during the third Quarter of Pregnancy. Indications and Results


Moez Kdous*, Oussema Khlifi, Marwen Braham, Jad Diari, Monia Ferchiou and Fethi Zhioua

Center of Reproductive Biology, Department of Gynecology and Obstetrics, Aziza Othmana Hospital, Tunis, University of Tunis El Manar, Tunisia.

Corresponding Author E-mail: kdousmoez@gmail.com

Accepted 7th July, 2015.



Abstract


Objective: We aimed to evaluate the benefit of achieving a late amniocentesis in presence of one or more sonographic signs, in patients who are not at high risk for T21 at the first and second trimesters of pregnancy. Methods: Descriptive retrospective study conducted over 6 years from January 2008 through December 2013 in 222 pregnant women who have had amniocentesis for fetal karyotyping after 28 weeks of amenorrhea (WA). Results: 306 amniocenteses for fetal karyotyping were analyzed. The mean age of the patients was 31 years (range: 17-45). Gestational age at amniocentesis was 32 WA (28-37 WA + 2 days). The mean interval between amniocentesis and delivery was 5 WA + 5 days. Nine (4.05%) cases of chromosome abnormalities were reported of which 6 (2.7%) were trisomy 21. The sonographic sign that generated the most amniocentesis was intrauterine growth restriction. The most correlated sign to trisomy was the atrioventricular canal defect. The two cases of polymalformative fetuses were related to trisomy 18. The karyotypes prepared in presence of a minor sonographic sign were all normal except for a partial trisomy 8 with dysmorphic corpus callosum on ultrasound. Conclusion: The third-trimester amniocentesis ensures the absence of chromosome abnormalities in patients initially classified at low risk during the first two trimesters of pregnancy, and in whom we discover late sonographic findings suggestive of aneuploidy. However, it must be applied only to specific situations and in countries where the legislation allows pregnancy medical termination of late stages. Although this practice has few complications, many of its indications should be reassessed.

Keywords: Trisomy 21, Amniocentesis, Fetal malformation, Screening.

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Cite This Article:

Moez Kdous*, Oussema Khlifi, Marwen Braham, Jad Diari, Monia Ferchiou and Fethi Zhioua. Amniocentesis for Fetal Karyotyping during the third Quarter of Pregnancy. Indications and Results. Donnish Journal of Medical Laboratory and Diagnosis 1(2) 2015 pp. 010-014.


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