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Donnish Journal of Medicine and Medical Sciences

April 2015 Vol. 2(3), pp. 026-031

Copyright © 2015 Donnish Journals




Original Research Paper


Species Typing with PCR–RFLP from Cutaneous Leishmaniasis Patients in Iraq


1Magda A. Ali, 2Abdulsadah A. Rahi and 3Ali Khamesipour*

1Department Microbiology, College of Medicine, Wasit University, Wasit, Iraq-Tehran University of Medical Sciences TUMS-IC, Tehran, Iran
2Department of Biology, College of Science, Wasit University, Kut, Iraq
3Center of Research and Training in Skin Diseases and Leprosy, TUMS, Tehran, Iran

Corresponding Author E-mail: ali.khamesipour@gmail.com

Accepted 4th April, 2015.



Abstract


A total of 20 skin lesion samples collected from patients with cutaneous leishmaniasis (CL) attended AL-Karamah Teaching Hospital at Wasit province. Dermal scrapings were analysed by examination of Giemsa-stained smears, by Conventional PCR and RFLP-PCR methods. The incidence rate with CL was 80% by using Giemsa-stained and 100% for both PCR and RFLP-PCR. Twelve (60%) of isolates were typed as L. major (LmA & LmB) and 8 (40%) were typed as L.tropica (LtA & LtB) by using HaeIII enzyme. The higher infection (45%) appeared in age group (1-12) years old and the wet type was predominance (65%).

Keywords: CL, RFLP-PCR, Giemsa

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Cite This Article:

Magda A. Ali, Abdulsadah A. Rahi and Ali Khamesipour. Species Typing with PCR–RFLP from Cutaneous Leishmaniasis Patients in Iraq. Donnish Journal of Medicine and Medical Sciences 2(3) 2015 pp. 026-031.


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