Donnish Journal of Medicine and Medical Sciences

May 2019 Vol. 5(1), pp. 001-008

Copyright © 2019 Donnish Journals

Original Research Article

The profiles of Ascl2 Gene Expression in different Conditions of Cells

M. Nurhalim Shahib¹*, Yussy Afriani Dewi², Vita Murniati Tarawan³, Achadiyani Agung Niyono⁴, Fenny Dwiyatnaningrum⁵, Diah Dhianawaty¹, Dzulfikar DLH⁵, Gatot Nyarumenteng³, and Andri Rezano⁶

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Padjadjaran.
²Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Padjadjaran.
³Department of Physiology and Anatomy, Faculty of Medicine, Universitas Padjadjaran.
⁴Department of Obstetric Obstetrics and Gynecology, Faculty of Medicine, Universitas Pad-jadjaran.
⁵Department of Pediatrics, Faculty of Medicine, Universitas Padjadjaran.
⁶Course Director, Graduate school of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran.

Corresponding Author's Email: nurhalimshahib@yahoo.com

Accepted 7th April, 2019.

Abstract

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The profiles of gene expressions are cellular responses to the several conditions varying from normal to pathologic. Although the Ascl2 expression increased in various cancers, however, its expression profiles are yet to be conclusively understood, such as changes in trophoblastic disease, nasopharyngeal, and ovarian cancer. By analyzing 3’end Ascl2 genes, both using the Sac-II restriction enzyme, RT-PCR, and single strand conformation polymorphism (SSCP), and DNA sequencing, we found that the 3’end of the Ascl2 gene also plays a role in gene expression in trophoblastic disease. It suggested, the expression of the Ascl2 gene is regulated by two regulators, one site from the 5’end and the other from 3’end. By using the experimental animals that were given treatment supplementation of soybean extract, resulting in the shortened villi and thicker crypt, suggesting Ascl2 had two different molecular targets. To implement the Ascl2 expression profile in medicine, we tested that gene in 258 healthy women (15-59 years). The results show that Ascl2 is a strong expression and the expression in adult women is stronger than in the young female. The expression of the Ascl2 gene in pathological conditions has been shown using nasopharyngeal cancer and ovarian cancer. As a comparison, we used a Hela cell. The results show that the expression of the Ascl2 in nasopharyngeal is stronger than in ovarian cancer (moderately expressed). In Hela cells, Ascl2 is expressed very strongly. To complete the profile, we also reported the miR302b, cMyc, and KLF4 gene expression. The different expression of Ascl2 on various cancer cells showed that cell cancers have their own mechanism dealing with the Ascl2 pathway. Therefore, it needs other genes such as cMyc, KLF4, and miR302b that completed the Ascl2 analysis..

Keywords: Threshold cycle, Ascl2, miR320b, Housekeeping gene.

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Cite This Article:

M. Nurhalim Shahib, Yussy Afriani Dewi, Vita Murniati Tarawan, Achadiyani Agung Niyono, Fenny Dwiyatnaningrum, Diah Dhianawaty1, Dzulfikar DLH, Gatot Nyarumenteng and Andri Rezano. The profiles of Ascl2 Gene Expression in different Conditions of Cells. Donnish Journal of Medicine and Medical Sciences 5(1) 2019 pp. 001-008.