Donnish Journal of Biomedical Research

November 2017 Vol. 4(1), pp. 001-005

ISSN: 2984-8954

Copyright © 2017 Donnish Journals

Original Research Paper

Lipopolysaccharide-induced Alterations of Immune-related Genes in Goat

Shahira Hussein¹, Abdelbary Prince²*, Adel Elbehairy², Nahla S Kotb³ and Manal Bahaa²

¹Unit of Immunology and Immunopharmacology, Animal Reproduction Research Institute, Agriculture Research Center of Egypt, 12556 Giza, Egypt.
²Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt.
³National Organization for Research and Control of Biological Product, Egypt.

Corresponding Author's Email: bioproteomics@yahoo.com

Accepted 15th November, 2017.

Abstract

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Background: Blood samples (8-10 ml) collected in heparinized sterile screw-capped tube from the jugular vein of twenty Egyptian goats were used in this study to be treated with Lipopolysaccharide (LPS) which is an endotoxin used as a model of inflammation. LPS offers an attractive model for inducing inflammation due to its ability to provoke secretion of tumor necrotic factor TNF-a and multiple cytokines (e.g. IL-1B, IL-6, and IL-10) so LPS is an important model used as an immune stimulant. The importance of endotoxin is based on a variety of biological responses which this molecule provokes both in vitro and in vivo. The biologically active moiety of endotoxin, the lipopolysaccharide (LPS) has been identified and biochemically characterized for a variety of enteric organisms. Further, the toxic principle of LPS lipid A has been defined and synthesized. Genes in LPS-stimulated monocytes is evaluated by quantitative real-time PCR using beta-actin for normalization. Results: Four genes (TNF-a, IL1-B, IL6 and IL10) were quantified by RT-qPCR in LPS-stimulated goat monocytes in vitro and viability of monocyte cells treated with LPS were evaluated using MTT assay resulted in decreased cell viability with increasing LPS concentrations in contrast to control group while there was an increase in gene expression of IL1-B, IL-6 and TNF-a but no change for IL10. Conclusions: LPS incubated with monocyte with different concentration resulted in decreased cell viability and increase gene expression.

Keywords: Toxin, Inflammation, Goats, Lipopolysaccharide.

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Cite This Article:

Shahira Hussein, Abdelbary Prince, Adel Elbehairy, Nahla S Kotb and Manal Bahaa. Lipopolysaccharide-induced Alterations of Immune-related Genes in Goat. Donnish Journal of Biomedical Research 4(1) 2017 pp. 001-005.