Donnish Journal of Genetics and Molecular Biology

April 2017 Vol. 2(2), pp. 005-010

Copyright © 2017 Donnish Journals

Original Research Paper

Noninvasive Markers for Detection of Nonalcoholic Fatty Liver Disease in Diabetic Egyptian Patients

Lamiaa Mobarak¹, Hams Ahmed Taha², Gamal Abo El Fath³, Maha Abdel Ghany⁴, Mona Nasar⁵, Hala H El Deeb⁵* and Nashwa Ahmed⁶

¹Tropical Medicine Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.
²Pediatric Department, Faculty of Medicine, Helwan University, Egypt.
³Pediatric Department, Gentic Unit, Faculty of Medicine, Ain Shams University, Egypt.
⁴Pediatric Department, El Sahel Teaching Hospital, Cairo, Egypt.
⁵Clinical Pathology Department, El Sahel Teaching Hospital. Cairo, Egypt.
⁶Clinical Pathology Department, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.

Accepted 4th March, 2017.

Abstract

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Nonalcoholic fatty liver disease (NAFLD), which is closely associated with obesity, has become the most common chronic liver disease in children and adolescents. The prevalence of pediatric NAFLD is 3% to 10% in normal-weight subjects and reaches a value of 80% in obese individuals. Patients and Methods: The present study included 200 diabetic patients developed to nonalcoholic fatty liver, recruited from Gastroenterology Outpatients Clinic in El Sahel Teaching Hospital. The study participants were divided into two groups: 100 healthy controls and 200diabetic patients with NAFLD (100 adults and 100 children). BMI: was calculated according to the following equation: weight (Kg) / height² (m²). Study population underwent fasting blood sampling to assess blood glucose (FBG), total cholesterol, HDL-cholesterol, triglycerides, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total and direct bilirubin, INR, alpha-fetoprotein (AFP) and creatinine by biochemical laboratory methods. Interleukin 6(IL6), Tumor necrosis factor (TNF-α) and f Monocyte Chemoattractant Protein-1 (MCP-1) were analyzed by ELIZA. Results: This study showed highest sensitivity (88%) at MCP-1 level 309 ng/ml and highest specificity when compared with IL6 and TNF- α respectively (80%, 80% and 72%, 65%). The cutoff value of MCP-1equal 309 ng/ml above which the patient will develop to NALFD.

Keywords: Nonalcoholic fatty liver disease, Interleukin 6, Tumor necrosis factor, Monocyte Chemoattractant Protein-1

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Cite This Article:

Lamiaa Mobarak, Hams Ahmed Taha, Gamal Abo El Fath, Maha Abdel Ghany, Mona Nasar, Hala H El Deeb and Nashwa Ahmed. Noninvasive Markers for Detection of Nonalcoholic Fatty Liver Disease in Diabetic Egyptian Patients. Donnish Journal of Genetics and Molecular Biology 2(2) 2017 pp. 005-010.