Donnish Journal of Genetics and Molecular Biology

February 2017 Vol. 2(1), pp. 001-004

Copyright © 2017 Donnish Journals

Original Research Paper

Molecular Study of two Types of Mutations in Promoters of IL-2 and IL-10 Genes in Iraqi Patients with Tuberculosis

Mazin S. Salman, Awatif H. Issa* and Majid N. Humoud

College of Sciences, University of Basra, Iraq.

Corresponding Author's E-mail: awatifhi@gmail.com

Accepted 20th January, 2017.

Abstract

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Tuberculosis remains as a black page in the ancient and modern history book of humanity. Mycobacterium tuberculosis is the main causative agent of TB in human. A defect in the genes of the immune response is the most plausible explanation for susceptibility of some people, and resistance of others to TB. Cytokines play a critical role in interactions and integration between the cells of immune system, which leads to effective defense against TB, among cytokines IL-2 and IL-10, which have regulatory role in immune response. The main goal of this study is to produce molecular analysis for promoters of IL-2 and IL-10 genes in Iraqi patients with TB. Seventy-four blood specimens were collected from 74 patients in the Institute of the Tuberculosis and Chest Disease–Basra city, blood specimens also collected from 74 healthy individuals as control. Extracted DNA was amplified using two sets of specific primers for promoter regions of IL-2 and IL-10 genes; purified amplicons were sequenced and were analyzed using specific software. Genotyping of IL-10 promoter displayed that range from 19 to 25 motifs but without 23-motif repeat. Individuals with CA21, CA22 and CA24 appeared to be the most susceptible to infection with (TB) with PIC larger than (0.5). CA20 is responsible for protective against tuberculosis, the t-value showed that there was significant differences between the patients and control groups, with PIC larger than (0.5). The results of the present study showed that there are two SNPs recorded in the amplified region of promoter of IL-2, which are A→C and T→G with frequencies of 0.1 and 0.19 respectively.

Keywords: Interleukin-2, Interleukin-10, Mycobacterium tuberculosis.

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Cite This Article:

Mazin S. Salman, Awatif H. Issa and Majid N. Humoud. Molecular Study of two Types of Mutations in Promoters of IL-2 and IL-10 Genes in Iraqi Patients with Tuberculosis. Donnish Journal of Genetics and Molecular Biology 2(1) 2017 pp. 001-004.