Donnish Journal of Medicine and Medical Sciences

February 2017 Vol. 4(2), pp. 008-013

Copyright © 2017 Donnish Journals

Original Research Article

Molecular Detection of Environmental Morganella morganii as Histamine Producing Bacteria

Hanaa K. Ibrahim¹, Abdulelah A. Almayah² and Awatif H. Issa³*

¹College of Veterinary Medicine, University of Basra, Iraq.
²College of Pharmacy, Department of Clinical Laboratory, University of Basra, Iraq.
³College of Sciences, Department of Biology, University of Basra, Iraq.

Corresponding Author's Email: awatifhi@gmail.com

Accepted 12th January, 2017.

Abstract

---

Histamine-producing bacteria may be associated with much irritable bowel disease. The present study included fresh samples of Carp fish muscles for histamine producing bacteria (HPB) detection. Preliminary detection was depended on Niven´s medium, out of 25 bacteria samples, only 17 species were positive for histamine production. Molecular technique by PCR, the responsible Histidine – Decarboxylase (HDC) 709 pb was detected by specific primer. The results were compactable and parallel as those in Niven´s medium detected for histamine production. The bacterial species were identified by 16SrDNA sequence technique. The specimens (36) were diagnosed by amplifying universal primer of 16SrDNA. Sequence data were analyzed by (ncbi) program. Sequence results showed dominance of Morganella morganii.

Keywords: Histamine, Morganella morganii, 16S rDNA.

Read: [Full Text - PDF]

Cite This Article:

Hanaa K. Ibrahim, Abdulelah A. Almayah and Awatif H. Issa. Molecular Detection of Environmental Morganella morganii as Histamine Producing Bacteria. Donnish Journal of Medicine and Medical Sciences 4(2) 2017 pp. 008-013.